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DIGITAL PCR ANALYSIS

3 Day Technical Hands-on Certificate Online Training in Droplet Digital PCR Data Analysis

4.7

Resource Person : Event Date :- 18 - 20 November, 2024. Event Timing :- New York 19:30 (PREVIOUS DAY) / London 00:30 / Paris 01:30 / Dubai 04:30 / New Delhi 6:00 / Singapore 08:30 / Sydney 11:30

This Courses Included

  • High demand video
  • Learn from Experts
  • Hands-on practical sessions
  • Certificate on completion

BDG LifeSciences is a distinguished bioinformatics company established in 2010 and operates globally. Headquartered in India the company specializes in facilitating workshops, training programs, novel & innovative research projects, and online courses in technologies of bioinformatics & life science. The company is registered under the Ministry of MSME (Micro, Small, and Medium Enterprises), Government of India, with the registration number UDYAM-UP-01-0019151. Recently, BDG Lifesciences, India has authorized BBR Group Pty Ltd., Australia (ACN 608 550 849), to operate its programs in Australia & New Zealand.
With a focus on practical application of technology, where participants work on their own computer/laptop on software/servers, BDG LifeSciences has been a leader in the sector for the last 14 years.
To view the pictures of our previous events click here-1 and click here-2. To view, the listing of upcoming events kindly click here and our previous workshops can be viewed here. To view the video feedback of our previous events kindly visit our YouTube Channel

On a huge demand we have launched our next 3-day complete hands-on Online Workshop in Droplet Digital PCR Data Analysis. This program will be an extensive hands-on training program in which everyday 120-150 minutes of live session will be conducted by Dr Shahul Hameed and participants will run the processes on their own computer systems with complete learning of this revolutionary technology. Video recording of each session will be provided at the end of the session to give the user a unique learning experience. On successful completion of the course participation certificate will be awarded.

 

INTRODUCTION

Droplet Digital PCR (ddPCR) is a novel method for nucleic acid quantification that allows for absolute quantification of DNA or RNA targets in a sample. Unlike traditional quantitative PCR (qPCR), which uses fluorescence signals to measure amplification of target nucleic acids, ddPCR partitions a sample into thousands of individual droplets, each serving as an individual reaction vessel. The amplification of the target nucleic acid is then carried out within each droplet, allowing for precise quantification of the target molecule. After amplification, the droplets are read to determine whether they contain amplified target molecules or not. This technology has become increasingly popular in recent years due to its high precision, sensitivity, and reproducibility, as well as its ability to detect rare targets with greater accuracy.

Droplet digital PCR has numerous applications in research and clinical settings. It is commonly used in cancer research to detect mutations, copy number variations, and gene fusions. It is also used in infectious disease research to quantify viral load and detect drug resistance mutations. Other applications include the detection of rare genetic disorders, gene expression analysis, and monitoring of gene editing and gene therapy. Additionally, ddPCR has the potential to be used in non-invasive prenatal testing and liquid biopsy for cancer detection. Overall, the high accuracy and sensitivity of ddPCR make it a powerful tool for a wide range of applications in biomedical research and diagnostics.

 

TOPICS

  • Analysis of copy number variations using droplet digital PCR
  • Quantification of gene expression using droplet digital PCR
  • Analysis of rare mutations using droplet digital PCR
  • Absolute quantification of viral load using droplet digital PCR
  • Assessment of chimerism in hematopoietic stem cell transplantation using droplet digital PCR
  • Quality control of gene editing using droplet digital PCR
  • Detection of circulating tumor DNA using droplet digital PCR
  • Comparison of droplet digital PCR and traditional qPCR for nucleic acid quantification
  • Detection of bacterial infections using droplet digital PCR
  • Determination of genetic risk factors for disease using droplet digital PCR.

OUTCOME OF THIS PROGRAM: This droplet digital PCR data analysis workshop can provide participants with a range of learning outcomes, including:

  • Understanding the principles of droplet digital PCR and its applications in biomedical research and diagnostics
  • Knowledge of the experimental design and data acquisition process for droplet digital PCR
  • Hands-on experience with the data analysis software, including data preprocessing, quality control, and normalization
  • Understanding of statistical methods for analyzing droplet digital PCR data, including hypothesis testing and regression analysis
  • Familiarity with open-source tools and software packages for droplet digital PCR data analysis
  • Interpretation of ddPCR data and visualization of results in a meaningful way
  • Ability to troubleshoot and optimize the experimental design for droplet digital PCR experiments
  • Understanding of best practices for reporting and presenting ddPCR data to the scientific community
  • Knowledge of current trends and challenges in droplet digital PCR data analysis and future directions for the technology.

Overall, the workshop can provide participants with the necessary skills and knowledge to analyze ddPCR data effectively and make significant contributions to biomedical research and diagnostics.

 

TIME- New York 19:30 (PREVIOUS DAY) / London 00:30 / Paris 01:30 / Dubai 04:30 / New Delhi 6:00 / Singapore 08:30 / Sydney 11:30

 

TARGET AUDIENCE

Student: Bachelor, Masters, PhD, MBBS, MD students as well as Faculty and Professors from Microbiology, Biochemistry, Biotechnology, Immunology, Medicine, Pharmacy, Pharmaceutical Chemistry, Biomedical Technology, Genetics, Bioinformatics, Plant Science and Life Sciences.

Professionals: Resident Doctors, Biotechnology, Bioinformatics, Medicine and Pharmaceutical scientists from industry, academia, and regulatory agencies. Hands-on exercises will be performed individually using software tools on your own laptops. (no prior experience required).

 

RESOURCE PERSON

Dr. Shahul Hameed is a researcher and his research involves developing CRISPR based gene therapy for Muscular dystrophy and Sickle Cell Anaemia. He is also involved in the development of novel delivery systems for gene therapy. His research interest extends to design of mRNA based therapeutics and design of Artificial Intelligence based Digital health & Imaging tools. In Collaboration with Dystrophy Annihilation Research Trust, his lab was involved in gene editing of DMD gene for development of CRISPR based therapeutics. [Nov 2018 to Aug 2022]

He has worked as Senior Assistant Professor at B.S. Abdur Rahman Crescent University, Chennai and earlier at Bannari Amman Institute of Technology, Erode. He was involved in teaching biotechnology subjects like Protein Engineering, Genomics and Proteomics, Structural Biology, Pharmaceutical Biotechnology, Genetic Engineering and Chemical Reaction Engineering to students. His research work involved Drug Design against S100A4 oncoprotein. Further development of Machine Learning based tools for predicting thermodynamic stability of enzyme mutants. He was selected through ERASMUS to conduct workshops on drug design and protein engineering at University of Nottingham, UK.

He is a PhD in Biological Sciences from Indian Institute of Science(IISc), Bangalore. At IISc, his research work involved Protein Engineering and Structural Characterization of the engineered mutants using Nuclear magnetic resonance spectroscopy and other biophysical methods. His research work also involved characterization of Protein-Protein interactions using experimental techniques, like NMR and Mass spectrometry, genetic engineering and Computer simulations.

He did his postdoctoral training at Tata Institute of Fundamental Research, Mumbai. There his work involved development of novel NMR techniques to study huge protein complexes. His work also involved Cloning, Protein Expression and Purification of motor proteins.

 

T & C

  • After registering, please email to info@bdglifesciences.com with your preferred start date and choose one of the following time slots: 6:30 AM–8:00 AM India time or 9:30 PM–11:00 PM India time.
  • Kindly note that the time of the sessions will be 60-90 minutes everyday. 
  • Once you register, please allow us 2-5 working days to make your training schedule,i.e. dates and time.
  • Please provide a GMAIL ID for registration as the recorded video session will be provided on YouTube. Kindly provide that email ID by which you use YouTube.
  • Since the training is a one-on-one live session with a trainer on Zoom, you'll need to attend, allowing for practical hands-on learning, real-time explanations, and direct query resolution for a better learning experience.
  • At the end of each session, you will receive a video recording, allowing you to review the steps as many times as needed to enhance your learning and master the topics and tasks.
  • BDG Lifesceiences (OPC) Private Limited is registered under Ministry of MSME(Micro, Small and Medium Enterprises), Government of India with Registration Number:UDYAM-UP-01-0019151 hence the certificate will be provided of it. BBR Group Pty Ltd, Australia (ACN-Australian Company Number-608 550 849) has the franchisee of BDG Lifesciences to run its programs in Australia & New Zealand.
  • The certificate will be issued as per the details which you will provide in the registration form while registering before payment.
  • We want to make sure that you learn properly hence the training certificate will be given ONLY on successful completion of all the tasks given by the trainer.
  • The certificates of all our Online programs are sent by email(softcopy) which has a unique barcode. You can take a print of that on heavy cardstock or photo paper and get it laminated if required.
  • The registration is NON-REFUNDABLE and NON-TRANSFERABLE.
  • BDG Lifesciences (OPC) Pvt. Ltd., India and BBR Group Pty Ltd Australia reserves the right of admission in all our programs.
  • If you are removed or your registration is canceled then there will be no answer to that. We have our own reasons for such an act of ours. If we do not wish to give this workshop to any participant then we will refund their amount. 
  • You should also read the Terms & Conditions page as well as the FAQs page.
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