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Single Cell RNASeq Research Project

Single-cell RNA-seq Analysis of Chemotherapy-Induced Transcriptomic Changes in Pancreatic Ductal Adenocarcinoma (PDAC)

4.7

Resource Person :

This Courses Included

  • High demand video
  • Learn from Experts
  • Hands-on practical sessions
  • Certificate on completion

INTRODUCTION

BDG LifeSciences' Research Project Training Program is a one-of-a-kind initiative designed to strengthen your profile and enhance career opportunities, whether for jobs or higher studies, by offering the chance to work on novel research projects using the latest technologies in bioinformatics. Since 2010, this program has addressed the critical need for high-quality publications by combining innovative teaching methods with practical applications. Conducted entirely online, it provides participants with the flexibility to choose session timings while saving on travel, accommodation, and food expenses. With over 88 research projects successfully completed and published at the international level, this program is ideal as a major or thesis project for final-year students or for those looking to advance their profiles.

Applications are now open for ONLY 4 SEATS in our 90th novel research project, titled "Single-cell RNA-seq Analysis of Chemotherapy-Induced Transcriptomic Changes in Pancreatic Ductal Adenocarcinoma (PDAC)" . The focus of this project is to analyze the impact of chemotherapy on the tumor microenvironment in pancreatic cancer using single-cell RNA sequencing data equipping undergraduates, graduates, and higher-degree holders with practical skills, real-world experience, and valuable publication opportunities.


Overview of the Project

Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most aggressive and treatment-resistant cancers, with limited therapeutic options. Chemotherapy is a mainstay of treatment, but its impact on the tumor microenvironment (TME) remains poorly understood. In this workshop, we will explore a research project modeled on a recent single-cell RNA sequencing (scRNA-seq) study that investigates the molecular and cellular alterations in PDAC tumors following chemotherapy.

Participants will work with real single-cell transcriptomic data derived from human PDAC tumors. The goal is to understand how treatment influences cell populations, gene expression, immune interactions, and potential therapeutic targets within the tumor ecosystem.

This project provides hands-on experience with modern computational biology techniques and enables students to carry out a full data analysis workflow, from count matrix preprocessing to biological interpretation.


Methodology

If you're passionate about cancer research, transcriptomics, and real-world data analysis, this program offers a unique opportunity to learn cutting-edge single-cell techniques and gain practical experience working with actual patient-derived datasets. This program uses a hands-on, project-based approach to single-cell RNA-seq analysis, guiding participants through a complete workflow—from loading count matrices to identifying tumor-specific pathways—using advanced tools like Seurat, SoupX, InferCNV, and CellPhoneDB in R.


What Are the Benefits of This Program

  • Gain hands-on experience with modern bioinformatics tools and real-world data
  • Learn how to process, analyze, and interpret single-cell RNA-seq datasets
  • Understand cancer biology, gene expression, and tumor microenvironment dynamics
  • Receive a certificate of completion to validate your skills
  • Access expert guidance and support throughout the project

How It Will Help in Your Career

This program equips you with in-demand skills in single-cell transcriptomics, bioinformatics analysis, and data interpretation—key areas in academic research, biotech, and pharma—giving you a competitive edge in job applications, graduate studies, or research roles.


Why Choose BDG LifeSciences for this Training?

With over a decade of experience delivering cutting-edge training worldwide, BDG LifeSciences blends scientific expertise with interactive learning to offer industry-relevant, practical education—trusted by students, researchers, and professionals across the globe.


Learning Outcomes

Literature Review:
Participants will engage in a comprehensive literature review, critically analyzing existing research relevant to bioinformatics. This stage emphasizes the importance of understanding the current state of knowledge, identifying gaps in research, and formulating questions that will guide subsequent stages of the project.

By the end of this research project, participants will be able to:

  • Analyze existing research relevant to bioinformatics
  • Understand NGS platform and transcriptome analysis
  • Load and preprocess single-cell RNA-seq data
  • Perform quality control and normalize gene expression data
  • Cluster cells and visualize data using dimensionality reduction techniques
  • Annotate cell types and identify malignant cell populations
  • Conduct differential gene expression analysis between treated and untreated tumors
  • Run pathway and gene set enrichment analyses
  • Investigate cell-cell communication using ligand-receptor interaction analysis
  • Summarize and present findings in a scientific format (slide deck)

Requirements: Basic Skill Requirement to be able to work on this project

  • R programming
  • Basic Linux command line

Objective:

To analyze how chemotherapy alters the tumor microenvironment (TME) in pancreatic cancer using publicly available single-cell RNA-seq data.


Tools Required:

  • R / RStudio
  • Seurat (v4+)
  • SoupX
  • InferCNV
  • CellPhoneDB
  • clusterProfiler / enrichR
  • GSEA

Project Modules

1. Loading Count Matrix and Metadata

  • Goal: Understand and load scRNA-seq count data
  • Learning Outcome: Ability to inspect and prepare raw scRNA-seq input for analysis

2. Quality Control and Filtering

  • Goal: Remove low-quality cells and potential doublets
  • Learning Outcome: Students can clean scRNA-seq data for analysis

3. Normalization, Scaling, and PCA

  • Goal: Prepare data for dimensionality reduction
  • Learning Outcome: Students understand preprocessing before clustering

4. Clustering and UMAP Visualization

  • Goal: Identify major cell types
  • Learning Outcome: Ability to visualize and interpret clusters

5. Cell Type Annotation

  • Goal: Label clusters based on known markers
  • Learning Outcome: Understand how to annotate cell types from scRNA-seq data

6. CNV Analysis to Identify Malignant Cells

  • Goal: Distinguish normal from malignant epithelial cells
  • Learning Outcome: Students learn CNV-based tumor identification

7. Differential Gene Expression Analysis

  • Goal: Compare gene expression between treated and untreated samples
  • Learning Outcome: Perform DGE and interpret gene signatures

8. Gene Set Enrichment Analysis (GSEA)

  • Goal: Understand pathway-level changes
  • Learning Outcome: Interpret high-level biological pathways

9. Ligand-Receptor Interaction Analysis

  • Goal: Discover cell-cell communication differences
  • Learning Outcome: Explore intercellular signaling in the TME

10. Reporting and Presentation

  • Goal: Prepare a research summary or poster
  • Learning Outcome: Translate analysis into scientific communication

T & C

  • System Requirements: The training and software installation require a system running Windows OS or Linux Or Mac.
  • Post-Registration Details: Upon successful registration and payment, you will receive a welcome email containing:
  • Instructions for system preparation and software installation.
  • A dataset of molecules for your project.
  • Relevant files, research papers, and materials.
  • Video Access: An introductory video will be included, providing an overview of the technology and covering the basics to advanced techniques used throughout the project.
  • Session Videos: After each training session, a recording of the same session will be provided for your reference.
  • Gmail/YouTube ID Requirement: Registration must be done using a Gmail ID or the email ID linked to your YouTube account. All videos will be uploaded privately on YouTube and can only be accessed by participants of the project.
  • Non-Refundable and Non-Transferable: Registration fees are strictly non-refundable and non-transferable under any circumstances.
  • Training Session Commitment: Once your system is prepared, inform us of your preferred training schedule. If you are unable to attend a scheduled session, the steps for that phase will be sent to you via email.
  • Certificate Eligibility: A certificate will only be awarded upon successful completion of all five project phases and assigned tasks.
  • Progress Monitoring: To ensure steady progress, you are required to submit a project report to your guide every fortnight for review and feedback.
  • Guide and Community Support: You will have direct access to your guide and a dedicated group of past participants, who are available to address your queries and provide timely support.

Must Read

The Research Project Training Program by BDG LifeSciences is not just a course but an investment in your future. The fee you pay guarantees unparalleled value, providing you with cutting-edge skills, real-world experience, and the opportunity to contribute to internationally published research. This program is designed to elevate your academic and professional profile, equipping you with expertise in bioinformatics and drug discovery that is highly sought after in today’s competitive job market.

By participating, you gain the chance to learn from seasoned experts, work on innovative projects, and create a strong foundation for careers in bioinformatics, pharmaceutical research, or higher education. The program’s comprehensive structure ensures you receive everything you need to succeed—training, practical application, resources, certifications, and networking opportunities.

Whether you aim to pursue advanced studies, secure a high-impact job, or become a leader in your field, this program opens doors to new possibilities and heights of success. With BDG LifeSciences, you are not just learning—you are building a future where your contributions to science and technology can make a real difference.


How To Register

To secure your spot:

  • Click on Register Now button and proceed.
  • For queries, contact us at research@bdglifesciences.com

Previous Events & Testimonials

- Gain insight into our past workshops:

  • Video Feedback: Visit our YouTube channel for testimonials.
  • Upcoming Programs: Explore other upcoming events here.

To get regular updates on Upcoming Job opportunities, Research Projects, Courses, Training and Workshops, kindly join our WhatsApp Channel /  Telegram

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  3. Shreya Mondal, B. Tech (Biotechnology), Amity University, Noida, India.
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  1. Bharti Mittal, PhD, Senior Scientist, Operations, MedGenome Labs Pvt Ltd, Bangalore, India.
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  5. Harshita Agarwal, B. Sc, Amity Institute of Biotechnology, Amity University, Noida , India.
  6. Gupta Radha Devi Shivprakash, Masters of Science in Bioinformatics, G.N Khalsa College, Matunga, Mumbai, India
  7. Raisa Dabhilkar, Masters of Science in Bioinformatics, G.N Khalsa College, Matunga, Mumbai, India.

53. Molecular Modelling study of phytoconstituents from medicinal plants of India | Discovery of natural anti-tubercular agents

  1. Taiba Hamed Youssef Gamal El Din, Master in Biotechnology & Life Science, Bani Sueif University, Bani Sueif, Egypt.
  2. Syeda Birjees Misbah, M.Sc., Biochemistry, REVA University, Bangalore, India.
  3. Aindrila Pal, B. Tech, Amity Institute of Biotechnology, Amity University, Noida, India.
  4. Preeti, M.Sc Biotech, Amity Institute of Biotechnology, Amity University, India.
  5. Ngoc Pham, The Applied Chemistry Department of the International University, Ho Chi Minh, Vietnam.
  6. Moumita Ganguly, Research Associate, IIT Mandi, India.
  7. Deeksha Gairola, M.Sc Biotech, Amity Institute of Biotechnology, Amity University, India.
  8. Ketaki Ghatole, B.E. Biotechnology, MS Ramaiah Institute of Technology, Bengaluru, India.

49. Molecular Modeling study of Zika Virus | Virtual Screening, Protein Modeling, Docking, ADMET and MD Simulations Study

  1. Dr. Alam El-Din, Hanaa Mahmoud, Virology and Immnunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
  2. Manjisa Choudhury, VIT University, Vellore, India.
  3. Nada Gamal Ibrahim El-Dawy, Faculty of Biotechnology, October University for Modern Sciences and Arts, Egypt (MSA University).

39. Study of derivatives of Chalcones as new Tyrosinase inhibitors: A Molecular Docking, ADME & Tox Study

  1. Adwaita Das, Department of Botany, The University of Burdwan, Burdwan, West Bengal, India
  2. Shreya John MSc Bioinformatics, St. Aloysius Institute of Management and Technology, Mangalore University.
  3. Lalit R. Samant, Project Assistant, Haffkine Institute for Training Research and Testing, India.
  4. K.C. Haritah Yadav, M. Sc (Medical Pharmacology) from Kamineni Institute of Medical Sciences, Narkrtpally, Nalgonda, Andhra Pradesh.
  5. Dr. N. Hari, School of Chemical & Biotechnology, SASTRA University, Thanjavur, Tamil Nadu.
  6. Dr(Mrs) Jhaumeer Laulloo Sabina, University of Mauritius, Reduit, Mauritius.

34. Study of extracts of Veratrum Dahuricum as potential Anti-tumor molecules: Molecular Docking & Modeling study with Farnesyl Pyrophosphate Synthase (FFPS)

  1. Gomathi Rajendran, ITC Zenith (M) Sdn. Bhd. (Conformity Assessment Body), Malaysia.
  2. Seetha Harilal, Department of Pharmacology, Nehru College of Pharmacy, Kerala University of Health Sciences, Thrissur, Kerala, India.
  3. Akshya Kumar Mailapali, I. M.Sc Bioinformatics , B.J.B. Autonomous College, Utkal University, Bhubaneshwar, India.
  4. Tanya Munjal, M.Sc Biotech, Amity Institute of Biotechnology, Amity University, India.
  5. Sambit Kumar Roy, M.Sc Biotech, Amity Institute of Biotechnology, Amity University, India.
  6. Lucky Gupta, M.Sc Biotech, Amity Institute of Biotechnology, Amity University, India.
  7. Gaurav Dutt, School of Basic and Applied Science, Dayananda Sagar University, Bangalore, India.
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