INTRODUCTION
BDG LifeSciences, incorporated in 2010 and located in India, is a world-renowned bioinformatics company. BDG LifeSciences, which specializes in workshops, training programs, creative research, and online courses in bioinformatics and life sciences, has been an expert in practical technological applications for over 14 years. The company is registered with the Ministry of MSME, Government of India, and has expanded its services into Australia and New Zealand through a collaboration with BBR Group Pty Ltd.
Since its establishment, BDG LifeSciences has effectively educated students, scientists, educators, and corporate personnel worldwide. We introduced CRISPR/Cas9 workshops in May 2017 and have continued to offer them successfully. Now advancing in the technology we have launched a 5- Day Technical Hands-on Certificate Online Workshop in INTRODUCTION OF CRISPR & IT'S APPLICATION IN GENE THERAPY. This program will cover the fundamentals of genome editing using CRISPR, with an emphasis on the computational components of the technology.
Course Overview
This 5-day hands-on online workshop will explore the cutting-edge field of CRISPR-based gene therapy. Participants will gain practical experience in designing CRISPR constructs, targeting specific genes, and evaluating outcomes for genetic disorders. The workshop will cover gene editing techniques, ethical considerations, and real-world applications of CRISPR in therapy.
TOPICS/MODULES COVERED IN THIS WORKSHOP:
Introduction to CRISPR-Cas9 and Gene Editing Fundamentals
- CRISPR-Cas9 Technology: Introduction to CRISPR-Cas9, its mechanism, and its applications in gene editing.
- CRISPR-Cas9 Components: Cas9 protein, guide RNA, PAM sequence, and DNA repair mechanisms.
- History and Development of CRISPR: Key milestones in CRISPR-based therapies.
- CRISPR-Cas9 Technology: Introduction to CRISPR-Cas9, its mechanism, and its applications in gene editing.
- Homology-Directed Repair (HDR) and Non-Homologous End Joining (NHEJ): Understanding DNA repair mechanisms post-editing.
- Overview of Gene Therapy: Understanding gene therapy and its applications in genetic disorders.
- Optimizing CRISPR Delivery: Methods for delivering CRISPR components into cells (e.g., viral vectors, nanoparticles).
Hands-on activity: Introduction to CRISPR design tools for designing guide RNAs (gRNAs).
CRISPR Design and Optimization
- Designing CRISPR Guide RNAs: Selecting target genes and designing effective guide RNAs for editing.
- Off-Target Prediction and Mitigation: Techniques for minimizing off-target effects and improving editing specificity.
Hands-on activity: Use of various tools to design and evaluate gRNAs for a specific target gene along with the off-target effects.
Gene Editing Techniques and Applications
- Gene Knockouts and Knock-ins: Methods for creating gene knockouts and inserting new genetic material.
- CRISPR Activation and Interference: Techniques for gene activation and repression without cutting DNA..
- Applications in Genetic Diseases: Exploring CRISPR-based therapies for conditions like sickle cell anemia, cystic fibrosis, and Duchenne muscular dystrophy.
Delivery Methods and Clinical Considerations
- In Vivo and Ex Vivo CRISPR Applications: Gene editing in living organisms versus isolated cells.
- CRISPR Delivery Vectors: Viral vectors (AAV, lentivirus) and non-viral delivery systems (lipid nanoparticles, electroporation).
- Ethical Considerations: Discussing ethical issues related to germline editing, consent, and clinical trials.
- Clinical Trials and Case Studies: Current status of CRISPR-based gene therapies in clinical trials.
Troubleshooting, Data Analysis, and Future Directions
- Troubleshooting CRISPR Experiments: Common challenges in CRISPR gene editing and how to address them.
- Emerging Technologies: Exploration of new gene editing technologies like CRISPR 2.0, epigenome editing, and gene drives.
- The Future of CRISPR Gene Therapy: Potential for curing genetic diseases, improving agriculture, and future clinical applications.
Learning Objectives
By the end of this workshop, participants will:
- Understand CRISPR-Cas9 Technology: Gain a comprehensive understanding of CRISPR-Cas9 mechanics, its components, and how gene editing works at the molecular level.
- Design Effective CRISPR Constructs: Learn how to design CRISPR guide RNAs (gRNAs) and vectors for specific gene targets, optimizing for high efficiency and low off-target effects.
- Apply Advanced CRISPR Techniques: Understand and implement advanced CRISPR techniques like base editing, prime editing, and CRISPR activation/repression.
- Optimize CRISPR Delivery Methods: Learn about various CRISPR delivery systems (e.g., viral vectors, nanoparticles) and apply these methods to design in vivo and ex vivo experiments.
- Explore Clinical Applications of CRISPR: Understand the current status of CRISPR-based gene therapies in clinical trials, including its potential to treat genetic disorders like sickle cell anemia and Duchenne muscular dystrophy.
- Consider Ethical Implications: Discuss ethical issues surrounding CRISPR-based gene therapies, including concerns about germline editing, consent, and long-term effects.
- Prepare for Real-World CRISPR Applications: Gain the practical skills and knowledge necessary to implement CRISPR technology for gene therapy research and clinical applications in the future.
Target Audience
This workshop is ideal for:
1. Students
- Including undergraduate and postgraduate students studying life sciences and technologies.
- Medical and pharmaceutical students who are exploring genetic engineering.
- Ph.D. candidates specializing in molecular biology, bioinformatics, or similar subjects.
2. Academicians
- Scholars looking to incorporate CRISPR knowledge into their coursework.
- Researchers intend to use CRISPR in experimental setups.
3. Industry Professionals:
- Students: Bachelor, Masters, PhD, MBBS, MD students as well as faculty and professors from Microbiology, Biochemistry, Biotechnology, Immunology, Medicine, Pharmacy, Pharmaceutical Chemistry, Biomedical Technology, Genetics, Bioinformatics, Plant Science and Life Sciences.
- Professionals: Resident doctors, bioinformatics, medicine, and pharmaceutical scientists from industry, academia and regulatory agencies.
Methodology
The program will be conducted through interactive online sessions that combine theoretical lectures with practical exercises using software like CRISPResso and SnapGene, and group discussions. Participants will engage in real-world simulations of gene editing and data analysis, ensuring an immersive hands-on learning experience.
Why you should attend:
Gain cutting-edge skills in gene editing, learn from industry experts, and enhance your expertise in CRISPR technology. This workshop offers practical training and insights into a rapidly evolving field with significant potential for medical and scientific advancements.
Why Choose BDG LifeSciences for CRISPR Training?
1. Experience and Credibility: BDG LifeSciences, with over 14 years of expertise, is a market leader in bioinformatics course training and workshops.
2. Global Reach: Programs are accessible by participants from all around the world, and the curriculum is customized to international standards.
3. Hands-On Learning: The emphasis on practical application ensures that participants may instantly apply their knowledge.
4. Expert Advice: Training is provided by professionals with substantial knowledge of bioinformatics and CRISPR technology.
How to Register
To secure your spot:
- Click on Register Now button and proceed.
- For queries, contact us at info@bdglifesciences.com
Previous Events and Testimonials
- Gain insight into our past workshops:
- Upcoming Programs: Explore other upcoming events here.
To get regular updates on Upcoming Job opportunities, Research Projects, Courses, Training and Workshops, kindly join our WhatsApp Channel / Telegram
T & C
- It is recommended to provide GMAIL email address.
- Video recording of each session will be provided at the end of the session to give the user a unique learning experience.
- Interactive training sessions will be conducted on Google Meet/Zoom so to give users a better learning experience.
- As it is with a LIVE TRAINER hence practical application, i.e., the experiments/tasks to be performed can be done in the best explainable manner.
- In this Online Workshop, there will be a LIVE trainer who will solve queries along with training.
- Make sure you register under the right category. If you register under the wrong category then your registration will be invalid and NO REFUND WILL BE MADE IN THIS CASE. This is your mistake and the company is not responsible for it.
- To avoid this do read the description of the ticket before selecting and proceeding with payment.
- You need to upload your professional ID which can be your Student ID Card or Research Institute ID Card or Company ID Card. DO NOT UPLOAD EXPIRED ID OR ANY GOVERNMENT ID LIKE AADHAR, PAN, you have to upload professional ID.
- The certificate will be issued as per the details which you provide in the registration form while registering before payment.
- Once you register relax we will send you the meeting link after the workshop registration gets over.
- The program is SELF-PACED.
- After each session video of that particular session will be shared with you so you can go through as many times as you want and perfect yourself in the topics & tasks.
- If you miss any session then NO PROBLEM you can still perform the tasks by going through the video of that particular session.
- We want to make sure that you learn properly hence the training certificate will be given ONLY on successful completion of all the tasks given by the trainer.
- The certificates of all our Online programs are sent by email (softcopy) which has a unique barcode. You can take a print of that on heavy cardstock or photo paper and get it laminated if required.
- The registration is NON-REFUNDABLE and NON-TRANSFERABLE.
- BDG Lifesciences reserves the right of admission in all our programs.
- If you are removed or your registration is canceled then there will be no answer to that. We have our own reasons for such an act of ours
- If we do not wish to give this workshop to any participant then we will refund their amount.
- You should also read the Terms & Conditions page as well as the FAQs page.
