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NGS | RNASeq | Transcriptomics

7-Day Certificate Online Training in NGS Data Analysis | RNASeq | Transcriptome Analysis [Reference (Alignment) based Gene Expression Analysis] on Windows, Linux & Mac OS

4.7

Resource Person : Event Date :- 30 January - 5 February, 2025. Event Timing :- Live trainer- Online sessions from 8:30 PM - 9:30 PM India time +5:30 GMT.

This Event Includes

  • High demand video
  • Learn from Experts
  • Hands-on practical sessions
  • Certificate on completion

Introduction

 

Develop your bioinformatics abilities with BDG LifeSciences' comprehensive 7-day online training program for Next-Generation Sequencing (NGS) data analysis, specifically RNA-Seq and transcriptome analysis. This hands-on session is designed for students, researchers, professors, pathologists, and scientists. It will provide you with the knowledge and practical experience you need to effectively analyse RNA sequencing results.

 


Course highlights:



1. Hands-On Practical Sessions: Participate in daily 60-minute interactive training sessions in which you will perform processes on your own computer, ensuring a practical learning experience.
2. Comprehensive Curriculum: Learn about NGS data analysis, including RNA-Seq processes, data preprocessing, alignment, quantification, differential expression analysis, and results interpretation.
3. Flexible Learning: You can participate from anywhere with a stable internet connection. Sessions are compatible with the Windows, Mac, and Linux operating systems.
4. Expert Advice: Learn from an experienced expert with a solid foundation in bioinformatics and computational biology.
5. Certificate of Completion: After successfully completing the course, you will receive a certificate that will enhance your professional qualifications.


Why should you attend this workshop?



In the age of high-throughput sequencing technology, the capacity to analyse and understand large-scale RNA-Seq data is critical for furthering genomics and transcriptomics research. This workshop provides a unique chance to:

1. Develop Practical Skills: Learn how to access and use bioinformatics tools and software required for RNA-Seq data analysis.
2. Understand RNA Sequencing Workflows: Gain understanding of the whole RNA-Seq analysis workflow, from raw data preprocessing to differential expression analysis.
3. Enhance research capabilities: Apply these strategies to your research projects to gain significant biological insights from transcriptome data.
4. Stay Current: Keep up with the most recent developments in NGS technologies and bioinformatics approaches.


Topics covered

Day 1: Basic Linux Command Line

Module 1: Introduction to Linux Command Line

  • Fundamentals of the Linux command line
  • Linux file systems and directory structures
  • File viewing and editing techniques
  • Essential commands: man, echo, ls, pwd, mkdir, rmdir, mv, cp, open, touch, cat, find, tail, wc, clear, diff

Day 2: Advanced Linux Commands and Shell Scripting

Module 2: Advanced Linux Commands

  • Text processing commands: grep, sed, awk
  • Data sorting and joining: cut, join, sort
  • Using specialized bioinformatics tools like Samtools and Bedtools
  • Working with biological data using command-line tools

Module 3: Shell Scripting for Automation

  • Introduction to Bash Sheel scripting
  • Variables, inputs, and outputs
  • If statements and comparisons
  • Conditional statements, loops & arithmetic operations
  • File handling and automation of repetitive tasks

Day 3: Introduction to NGS and Sequencing Methods

Module 4: Overview of Next-Generation Sequencing (NGS)

  • History and evolution of sequencing technologies
  • 1st, 2nd and  3rd generation sequencing
  • Workflow of high-throughput sequencing

Module 5: Sequencing Methods

  • Pyrosequencing techniques
  • Sequencing by synthesis and ligation methods
  • Ion semiconductor sequencing approaches

Day 4: RNA-Seq Basics and Terminology

Module 6: Introduction to RNA-Seq

  • Fundamentals of RNA-Seq and its applications
  • Gene expression profiling and analysis
  • Coverage concepts—sequencing depth and breadth
  • Coverage calculation using Lander-Waterman equation

Day 5: Genomic File Formats and Quality Control

Module 7: Understanding Genomic File Formats

  • Overview of FASTA, FASTQ, BAM, SAM   and CRAM formats
  • Application and interpretation of genomic data

Module 8: Data Retrieval and Quality Control

  • Data collection from databases like NCBI SRA
  • Assessing data quality using FastQC
  • Trimming and filtering techniques for preprocessing

Day 6: Data Alignment and Genome Mapping

Module 9: Alignment Techniques

  • Genome assembly and annotation basics
  • Formats for annotation data (GTF, GFF)
  • Read alignment with HISAT2 and STAR tools

Day 7: Statistical Analysis and DEG Analysis

Module 11: Statistical Analysis Techniques

  • Normalization methods (TPM, RPKM, FPKM)
  • Principal Component Analysis (PCA) and clustering techniques

Module 12: Differential Gene Expression (DEG) Analysis

  • Quantification of gene expression
  • Identification of differentially expressed genes

Who should attend?


1. Students and researchers in life science, bioinformatics, and computational biology.
2. Biologists are striving to improve their data analysis skills.
3. Industry professionals involved in NGS data or transcriptome analysis.
4. Beginners who are interested in genomics and RNA-Seq technology.


Takeaways from the Workshop


1. Experience with Linux commands and scripting for bioinformatics.
2. Proficiency with NGS processes and RNA-Seq data processing.
3. Ability to undertake statistical and differential gene expression analyses.
4. Gain hands-on experience with industry-standard tools including HISAT2, STAR, and FastQC.
5. Ability of handling large-scale sequencing datasets with confidence.


Why Choose BDG LifeSciences?

Since 2010, BDG LifeSciences is leading the way in bioinformatics and life sciences education. With more than 14 years of expertise, we have trained thousands of participants worldwide, assisting them in becoming successful in both industry and academics.

  Our courses emphasize real-world, application-based learning, guaranteeing that students acquire practical experience in their chosen field.  By choosing this training , you are investing in high-quality education from highly qualified professionals.

Take the first step towards learning the exciting fields of molecular biology and genetic engineering.  Register and reserve your position in this revolutionary program.
  Spaces are limited, so don't delay in acquiring the skills and knowledge required to foster scientific innovation from our bioinformatics course


Join Us

This NGS RNA-Seq and Transcriptome Analysis workshop provides an exclusive chance to learn how to analyse high-throughput sequencing data. Participants will get relevant ideas and tools to advance their genomics research through a combination of theoretical seminars and practical exercises.

Don't miss this opportunity to improve your bioinformatics skills! Register now and take the first step towards realising the potential of transcriptomics.


How to Register

To secure your spot:

  • Visit the official registration page
  • For queries, contact us at  info@bdglifescience.com

Previous Events and Testimonials

Gain insight into our past workshops:

  • Photo Gallery: Check out event pictures
  • Video Feedback: Visit our YouTube chgeannel for testimonials.
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