INTRODUCTION
BDG LifeSciences is a distinguished bioinformatics company established in 2010 and operates globally. Headquartered in India the company specializes in facilitating workshops, training programs, novel & innovative research projects, and online courses in technologies of bioinformatics & life science. The company is registered under the Ministry of MSME (Micro, Small, and Medium Enterprises), Government of India, with the registration number UDYAM-UP-01-0019151. Recently, BDG Lifesciences, India has authorized BBR Group Pty Ltd., Australia (ACN 608 550 849), to operate its programs in Australia & New Zealand.
With a focus on practical application of technology, where participants work on their own computer/laptop on software/servers, BDG LifeSciences has been a leader in the sector for the last 14 years.
Since its inception, BDG LifeSciences has successfully educated a diverse range of participants globally, including students, scientists, faculty members, professors, and corporate executives.
Innovation & creativity is an integral part of BDG Lifesciences and we always launch new programs on the newest topics. We were the first to launch a workshop and training course (Online & Onsite) on CRISPR/Cas 9 in May 2017 and since then doing in it successfully. Now advancing in the technology we have launched a 5-day complete hands-on Online Training Program in CRISPR GENE THERAPY.
This 5-day hands-on online workshop will explore the cutting-edge field of CRISPR-based gene therapy. Participants will gain practical experience in designing CRISPR constructs, targeting specific genes, and evaluating outcomes for genetic disorders. The workshop will cover gene editing techniques, ethical considerations, and real-world applications of CRISPR in therapy.
Course Overview
TOPICS/MODULES COVERED IN THIS WORKSHOP:
Introduction to CRISPR-Cas9 and Gene Editing Fundamentals
- Overview of Gene Therapy: Understanding gene therapy and its applications in genetic disorders.
- CRISPR-Cas9 Technology: Introduction to CRISPR-Cas9, its mechanism, and its applications in gene editing.
- CRISPR-Cas9 Components: Cas9 protein, guide RNA, PAM sequence, and DNA repair mechanisms.
- History and Development of CRISPR: Key milestones in CRISPR-based therapies.
Hands-on activity: Introduction to CRISPR design tools like Benchling or Geneious Prime for designing guide RNAs (gRNAs).
CRISPR Design and Optimization
- Designing CRISPR Guide RNAs: Selecting target genes and designing effective guide RNAs for editing.
- Off-Target Prediction and Mitigation: Techniques for minimizing off-target effects and improving editing specificity.
- Homology-Directed Repair (HDR) and Non-Homologous End Joining (NHEJ): Understanding DNA repair mechanisms post-editing.
- Optimizing CRISPR Delivery: Methods for delivering CRISPR components into cells (e.g., viral vectors, nanoparticles).
Hands-on activity: Use CRISPResso or CHOPCHOP to design and evaluate gRNAs for a specific target gene.
Gene Editing Techniques and Applications
- Gene Knockouts and Knock-ins: Methods for creating gene knockouts and inserting new genetic material.
- CRISPR Activation and Interference: Techniques for gene activation and repression without cutting DNA.
- Base Editing and Prime Editing: Advances in precision editing, including base editing to correct point mutations.
- Applications in Genetic Diseases: Exploring CRISPR-based therapies for conditions like sickle cell anemia, cystic fibrosis, and Duchenne muscular dystrophy.
Hands-on activity: Perform a CRISPR knock-in experiment simulation using Benchling or Geneious Prime.
Delivery Methods and Clinical Considerations
- In Vivo and Ex Vivo CRISPR Applications: Gene editing in living organisms versus isolated cells.
- CRISPR Delivery Vectors: Viral vectors (AAV, lentivirus) and non-viral delivery systems (lipid nanoparticles, electroporation).
- Ethical Considerations: Discussing ethical issues related to germline editing, consent, and clinical trials.
- Clinical Trials and Case Studies: Current status of CRISPR-based gene therapies in clinical trials.
Hands-on activity: Analyze and compare different CRISPR delivery methods using available online databases and tools.
Troubleshooting, Data Analysis, and Future Directions
- Troubleshooting CRISPR Experiments: Common challenges in CRISPR gene editing and how to address them.
- Analyzing CRISPR Results: Techniques for evaluating gene editing efficiency, off-target effects, and validation methods.
- Emerging Technologies: Exploration of new gene editing technologies like CRISPR 2.0, epigenome editing, and gene drives.
- The Future of CRISPR Gene Therapy: Potential for curing genetic diseases, improving agriculture, and future clinical applications.
Hands-on activity: Analyze CRISPR editing efficiency using Sanger sequencing data and CRISPResso for indel analysis.
Practical Application
SOFTWARE & SERVERS WHICH WILL BE USED:
Benchling
- Used for designing and optimizing CRISPR guide RNAs (gRNAs), designing gene editing experiments, and tracking experimental workflows.
Geneious Prime
- Employed for CRISPR gRNA design, sequence analysis, and visualization of gene editing results.
CRISPResso
- Tool for analyzing CRISPR editing outcomes, including off-target effects and editing efficiency, with visualization of indels.
CHOPCHOP
- Online tool used to design CRISPR guide RNAs, evaluate off-target effects, and optimize gRNA sequences.
Sanger Sequencing Software
- For analyzing sequencing data to verify the success and efficiency of CRISPR editing.
SnapGene
- Used for designing and simulating CRISPR gene editing experiments and visualizing the resulting gene sequences.
Vector NTI
- Software for designing, cloning, and simulating CRISPR vectors, as well as for gene synthesis and primer design.
BLAST (Basic Local Alignment Search Tool)
- Used to check for potential off-target sequences by aligning gRNA sequences with the genome.
TIDE (Tracking of Indels by Decomposition)
- Analyzes CRISPR-induced mutations from sequencing data to measure editing efficiency and accuracy.
GeneArt
- Tool for designing and synthesizing CRISPR constructs, and optimizing CRISPR sequences.
AAV (Adeno-Associated Virus) Vector Design Tools
- For designing CRISPR delivery vectors, specifically AAV-based delivery systems used for in vivo gene editing.
Learning Objectives
OUTCOME [WHAT PEOPLE WILL LEARN]:
By the end of this workshop, participants will:
- Understand CRISPR-Cas9 Technology: Gain a comprehensive understanding of CRISPR-Cas9 mechanics, its components, and how gene editing works at the molecular level.
- Design Effective CRISPR Constructs: Learn how to design CRISPR guide RNAs (gRNAs) and vectors for specific gene targets, optimizing for high efficiency and low off-target effects.
- Evaluate Gene Editing Outcomes: Develop skills to analyze CRISPR editing outcomes using tools like CRISPResso, TIDE, and Sanger sequencing for measuring editing efficiency and identifying off-target effects.
- Apply Advanced CRISPR Techniques: Understand and implement advanced CRISPR techniques like base editing, prime editing, and CRISPR activation/repression.
- Optimize CRISPR Delivery Methods: Learn about various CRISPR delivery systems (e.g., viral vectors, nanoparticles) and apply these methods to design in vivo and ex vivo experiments.
- Troubleshoot and Validate CRISPR Experiments: Gain troubleshooting skills for common challenges in CRISPR gene editing, and learn how to validate gene edits using sequencing and molecular assays.
- Explore Clinical Applications of CRISPR: Understand the current status of CRISPR-based gene therapies in clinical trials, including its potential to treat genetic disorders like sickle cell anemia and Duchenne muscular dystrophy.
- Consider Ethical Implications: Discuss ethical issues surrounding CRISPR-based gene therapies, including concerns about germline editing, consent, and long-term effects.
- Learn Data Analysis and Visualization: Master bioinformatics tools and software (e.g., Geneious Prime, Benchling, CRISPResso) for designing, analyzing, and visualizing CRISPR experiments.
- Prepare for Real-World CRISPR Applications: Gain the practical skills and knowledge necessary to implement CRISPR technology for gene therapy research and clinical applications in the future.
DURATION [IN DAYS & TIME SPENT FOR EVERYDAY SESSION]: 5 days (2 hrs / day)
SYSTEM REQUIREMENT: Laptop or PC with minimum 4GB RAM
PRE-REQUISITES [ANY PRIOR KNOWLEDGE REQUIRED]: Not Needed
Target Audience
This workshop is ideal for:
1. Students
- Including undergraduate and postgraduate students studying life sciences and technologies.
- Medical and pharmaceutical students who are exploring genetic engineering.
- Ph.D. candidates specializing in molecular biology, bioinformatics, or similar subjects.
2. Academicians
- Scholars looking to incorporate CRISPR knowledge into their coursework.
- Researchers intend to use CRISPR in experimental setups.
3. Industry Professionals:
- Students: Bachelor, Masters, PhD, MBBS, MD students as well as faculty and professors from Microbiology, Biochemistry, Biotechnology, Immunology, Medicine, Pharmacy, Pharmaceutical Chemistry, Biomedical Technology, Genetics, Bioinformatics, Plant Science and Life Sciences.
- Professionals: Resident doctors, bioinformatics, medicine, and pharmaceutical scientists from industry, academia and regulatory agencies.
Methodology
The program will be conducted through interactive online sessions that combine theoretical lectures with practical exercises using software like CRISPResso and SnapGene, and group discussions. Participants will engage in real-world simulations of gene editing and data analysis, ensuring an immersive hands-on learning experience.
Why You Should Attend
Gain cutting-edge skills in gene editing, learn from industry experts, and enhance your expertise in CRISPR technology. This workshop offers practical training and insights into a rapidly evolving field with significant potential for medical and scientific advancements.
Why Choose BDG LifeSciences for this Training?
1. Experience and Credibility: BDG LifeSciences, with over 14 years of expertise, is a market leader in bioinformatics course training and workshops.
2. Global Reach: Programs are accessible by participants from all around the world, and the curriculum is customized to international standards.
3. Hands-On Learning: The emphasis on practical application ensures that participants may instantly apply their knowledge.
4. Expert Advice: Training is provided by professionals with substantial knowledge of bioinformatics and CRISPR technology.
How To Register
To secure your spot:
- Click on Register Now button and proceed.
- For queries, contact us at info@bdglifesciences.com
Previous Events & Testimonials
- Gain insight into our past workshops:
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T & C
- It is recommended to provide GMAIL email address.
- Video recording of each session will be provided at the end of the session to give the user a unique learning experience.
- Interactive training sessions will be conducted on Google Meet/Zoom so to give users a better learning experience.
- As it is with a LIVE TRAINER hence practical application, i.e., the experiments/tasks to be performed can be done in the best explainable manner.
- In this Online Workshop, there will be a LIVE trainer who will solve queries along with training.
- Make sure you register under the right category. If you register under the wrong category then your registration will be invalid and NO REFUND WILL BE MADE IN THIS CASE. This is your mistake and the company is not responsible for it.
- To avoid this do read the description of the ticket before selecting and proceeding with payment.
- You need to upload your professional ID which can be your Student ID Card or Research Institute ID Card or Company ID Card.
- The certificate will be issued as per the details which you provide in the registration form while registering before payment.
- Once you register relax we will send you the meeting link after the workshop registration gets over.
- The program is SELF-PACED.
- After each session video of that particular session will be shared with you so you can go through as many times as you want and perfect yourself in the topics & tasks.
- If you miss any session then NO PROBLEM you can still perform the tasks by going through the video of that particular session.
- We want to make sure that you learn properly hence the training certificate will be given ONLY on successful completion of all the tasks given by the trainer.
- The certificates of all our Online programs are sent by email (softcopy) which has a unique barcode. You can take a print of that on heavy cardstock or photo paper and get it laminated if required.
- The registration is NON-REFUNDABLE and NON-TRANSFERABLE.
- BDG Lifesciences reserves the right of admission in all our programs.
- If you are removed or your registration is canceled then there will be no answer to that. We have our own reasons for such an act of ours
- If we do not wish to give this workshop to any participant then we will refund their amount.
- You should also read the Terms & Conditions page as well as the FAQs page.
