INTRODUCTION

BDG LifeSciences is a distinguished bioinformatics company established in 2010 and operates globally. Headquartered in India, the company specializes in facilitating workshops, training programs, novel and innovative research projects, and online courses in bioinformatics and life sciences. BDG LifeSciences is registered under the Ministry of MSME (Micro, Small, and Medium Enterprises), Government of India, with the registration number UDYAM-UP-01-0019151. Recently, BDG LifeSciences, India, has authorized BBR Group Pty Ltd., Australia (ACN 608 550 849), to operate its programs in Australia and New Zealand.

With a strong focus on the practical application of technology, BDG LifeSciences provides hands-on training where participants work on their own computers/laptops using specialized software and servers. The company has been a leader in this sector for the last 14 years, successfully educating a diverse range of participants, including students, scientists, faculty members, professors, and corporate executives worldwide.

Innovation & creativity is an integral part of the BDG Lifesciences and we always launch new programs on the newest topics. We have launched a 15-day Technical Hands-on Training Program in Next Generation Sequencing & RNA-Seq Data Analysis . It will be an extensive 15-day training program in which every day 60 minutes interactive training sessions will be conducted to give the user a unique learning experience. VIDEO RECORDING WILL BE PROVIDED. On successful completion of the course participation certificate will be awarded.

As it is 100% hands-on training program participants will run the processes on their own computer systems having Windows/ Mac/ Linux with a unique learning experience. The program will be of great help to students/ researchers/ faculty/ pathologists/ scientists in learning this latest technology.

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Why This Course is Important?

This course provides essential knowledge and hands-on skills for mastering bioinformatics workflows, specifically in the context of next-generation sequencing (NGS) data analysis. With the rise of high-throughput technologies, the ability to process, analyze, and interpret large datasets is becoming crucial in biological research. Mastering command-line tools, scripting, and NGS techniques empowers researchers to handle vast amounts of genomic data more efficiently. RNA-Seq (RNA sequencing) is a powerful tool for transcriptome analysis, enabling researchers to study gene expression patterns across different tissues, conditions, or time points. By sequencing the total RNA from a sample, RNA-Seq provides a comprehensive view of the transcriptome, allowing for the quantification of gene expression, the identification of novel transcripts, and the detection of alternative splicing events.

Research Highlights RNA-Seq Analysis

1. Gene expression patterns
2. Identify genes that are significantly upregulated or downregulated in response to a specific condition
3. Identification of novel transcripts
4. Gene Regulation

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Schedule

	Module Name	Topics
Day 1	Module 1: Basic Linux command line	Intro to the command line Linux file system Viewing and editing files basic commands: man, echo, ls, pwd, mkdir, rmdir, mv, cp, open, touch, cat, find, tail, wc, clear, diff
Day 2	Module 2: Advance Linux commands	Use of vim, grep, less, cut, join, sort, sed, awk command Use of samtools, bedtools commands Using the above commands to handle the biological data
Day 3	Module 3: Shell scripting	Introduction to Bash Shell Scripting Variables and read user inputs If statements and comparisons Positional parameters and conditional loops Functions and arithmetics File conditions
Day 4	Module 4: Introduction to NGS	NGS overview Evolution of sequencing: 1st, 2nd& 3rd generation sequencing High throughput sequencing workflow
Day 5	Module 5: Sequencing methods	Pyrosequencing sequencing by synthesis sequencing by ligation ion semiconductor sequencing
Day 6	Module 6: Introduction to RNA seq and Basic Terminology	Overview of RNA-Seq and its applications Gene expression data Sequencing coverage, Breadth of coverage Coverage calculation: Lander-waterman equation
Day 7	Module 7: Genomics file format	FASTA, FASTQ, BAM/SAM/CRAM
Day 8	Module 8: Database & Quality Control	Data Retrieval (NCBI SRA) & Data types Read Quality Check (FastQC) Trimming and filtering
Day 9	Module 9: Alignment	Genome assembly Annotation (GTF, GFF file format) Alignment of reads using reference Genome (HISAT2 / STAR)
Day 10	Module 10: UCSC genome browser	Visualization of mapped reads (UCSC & IGV)
Day 11	Module 11: Statistical Analysis	Normalization techniques (TPM, RPKM, FPKM) PCA, clustering
Day 12	Module 12: DEG analysis	Gene Expression Quantification Differential expression analysis
Day 13	Module 13: Functional Analysis	Pathway & Gene ontology enrichment analysis
Day 14	Module 14: Visualization	Different plots (Heatmap,volcano plot, box plot, etc) using R
Day 15	Module 15: Doubt clarification	Clarifying doubts and questions on a mini project

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Pre-requisites

• Basic knowledge of R.

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Learning Outcome: What will I learn? After this course, you should be able to:

After this course, you should be able to: 

1. Navigate the Linux command line and perform file management tasks.
2. Use advanced Linux commands and tools like samtools and bedtools for biological data analysis.
3. Write and execute shell scripts to automate data processing.
4. Understand the workflow of next-generation sequencing and key sequencing methods.
5. Interpret various genomics file formats (FASTA, FASTQ, BAM/SAM).
6. Perform data retrieval, quality control, and read trimming.
7. Align sequencing reads to reference genomes and annotate them.
8. Conduct statistical analysis
9. Carry out differential gene expression and functional enrichment analyses.
10. Repeat the course analysis on your data to do end-to-end analysis

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Target Audience

Student & Faculty: Bachelor, Masters, Ph.D., students as well as Faculty and Professors from Microbiology, Biochemistry, Biotechnology, Immunology, Pharmacy, Pharmaceutical Chemistry, Biomedical Technology, Genetics, Bioinformatics, Plant Science and Life Sciences.

Professionals: Biotechnology, Bioinformatics and Pharmaceutical scientists from industry, academia, and regulatory agencies. Hands-on exercises will be performed individually using software tools (no prior experience required).

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How To Register

To secure your spot:

• Click on Register Now button and proceed.
• After registering, please email to info@bdglifesciences.com with your preferred start date and choose one of the following time slots: 6:30 AM–8:30 AM India time or 8:30 PM–10:30 PM India time.
• Kindly note that the time of the sessions will be 90-120 minutes every day. 
• Once you register, please allow us 2-5 working days to make your training schedule, i.e. dates and time.
• For any further queries, feel free to email us at info@bdglifesciences.com

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Previous Events & Testimonials

Gain insight into our past trainings:

• Video Feedback: Visit our YouTube channel for testimonials.
• Upcoming Training Programs: Explore other upcoming events here.
• Upcoming Online Workshops: Explore other upcoming events here.

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To get regular updates on Upcoming Job opportunities, Research Projects, Courses, Training and Trainings, kindly join our WhatsApp Channel /  Telegram

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T & C

• Please provide a GMAIL ID for registration as the recorded video session will be provided on YouTube. Kindly provide that email ID by which you use YouTube.
• Since the training is a one-on-one live session with a trainer on Zoom, you'll need to attend, allowing for practical hands-on learning, real-time explanations, and direct query resolution for a better learning experience.
• At the end of each session, you will receive a video recording, allowing you to review the steps as many times as needed to enhance your learning and master the topics and tasks.
• BDG Lifesceiences (OPC) Private Limited is registered under Ministry of MSME(Micro, Small and Medium Enterprises), Government of India with Registration Number:UDYAM-UP-01-0019151 hence the certificate will be provided of it. BBR Group Pty Ltd, Australia (ACN-Australian Company Number-608 550 849) has the franchisee of BDG Lifesciences to run its programs in Australia & New Zealand.
• The certificate will be issued as per the details which you will provide in the registration form while registering before payment.
• We want to make sure that you learn properly hence the training certificate will be given ONLY on successful completion of all the tasks given by the trainer.
• The certificates of all our Online programs are sent by email (softcopy) which has a unique barcode. You can take a print of that on heavy cardstock or photo paper and get it laminated if required.
• The registration is NON-REFUNDABLE and NON-TRANSFERABLE.
• BDG Lifesciences (OPC) Pvt. Ltd., India and BBR Group Pty Ltd Australia reserve the right of admission in all our programs.
• If you are removed or your registration is canceled then there will be no answer to that. We have our own reasons for such an act of ours. If we do not wish to give this training to any participant then we will refund their amount.
• You should also read the Terms & Conditions page as well as the FAQs page. For any assistance kindly chat with our AI Assistant BioBot on the website www.bdglifesciences.com